Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
|Published (Last):||10 May 2017|
|PDF File Size:||3.6 Mb|
|ePub File Size:||18.87 Mb|
|Price:||Free* [*Free Regsitration Required]|
This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs eoena Primers sequences, genome positions and the size of PCR products are shown in Table 1.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.
Open in a separate window.
The polymerase auneszky reaction PCR is a rapid tool that can ed used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection. Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract.
The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8. Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.
Doença de Aujeszky
The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. This suibos possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR.
The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products.
Agarose gel electrophoresis was used to detect PCR products. The polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 there is no standard procedure recommended so far 2.
The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome Primers designed for the specific amplification of the viral gD glycoprotein gene odena the PRV genome.
Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.
Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for Aujeszzky genomes.
Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.
In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. Can J Com Med. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.
Iowa State University Press; Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated.
The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived ayjeszky seven healthy pigs. Published online Sep 1. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. Thus, the optimal concentration of MgCl2 and primers were 1. This region was highly conserved for all reported genomes as shown by aligning of these sequences.
The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.